amandarigdon
The Nerd Perspective

‘Instant’ Cannabis Potency Testing: Different Approaches from Different Manufacturers

By Amanda Rigdon
5 Comments
amandarigdon

An inside look at SRI Instruments and PerkinElmer, and the instrumentation they offer.

This is the first piece of a regular column that CIJ has been so kind to allow me to write for their publication. Some readers might recognize my name from The Practical Chemist column in this publication. Since the inception of that column, I’ve finally taken the plunge into the cannabis industry as chief technical officer of Emerald Scientific. Unlike The Practical Chemist, I will not spend the entire first article introducing the column. The concept is simple: while I find the textbook-esque content of The Practical Chemist scintillating, I have a feeling that the content is a little too heavy to spring on someone who is looking for engaging articles over their precious coffee break. Instead, The Nerd Perspective will consist of less-technical writing focusing on my experience and insights for the cannabis industry as a whole. But don’t worry – I’m sure I will not be able to refrain from technical jargon altogether.

To kick off the column, I want to talk about instrumentation for ‘instant’ cannabis potency testing. At this point, it’s common knowledge in the cannabis analytics industry that the most accurate way to test cannabis potency is through extraction then analysis by HPLC-UV. I agree wholeheartedly with that sentiment, but HPLC analyses have one drawback: they can be either inexpensive or fast – not both. There are some instruments entering the market now that– while not as directly quantitative as HPLC-UV – promise to solve the inexpensive/fast conundrum. During my most recent trip to California, I was able to spend some quality time with two well-known instrument manufacturers: SRI Instruments and PerkinElmer, both of whom manufacture instruments that perform fast, inexpensive cannabis potency analyses. From my previous home at the heights of The Ivory Tower of Chromatography: Home of the Application Chemists, SRI and PE couldn’t be more different. But as seen through the eyes of a company who deals with a wide range of customers and analytical needs, it turns out that SRI and PE are much the same – not only in their open and honest support of the cannabis industry, but also in terms of their love of all things technical.

My first stop was SRI Instruments. They are a relatively small company located in an unassuming building in Torrance, CA. Only a few people work in that location, and I spent my time with Hugh Goldsmith (chief executive officer) and Greg Benedict (tech service guru). I have worked with these guys for a few years now, and since the beginning, I have lovingly referred to them as the MacGyvers of chromatography. Anyone familiar with SRI GCs knows that what they lack in aesthetics, they make up for in practicality – these instruments truly reflect Hugh and Greg’s character (that’s meant as a compliment).

SRI specializes in relatively inexpensive portable and semi-portable instruments that are easy to set up, easy to operate, and most importantly – engineered for a purpose. It’s actually really hard to manufacture an instrument that meets all three of these criteria, and the folks at SRI accomplish this with their passionate and unique approach to problem solving. What I love about these guys is that for them, nothing is impossible. Here’s an example: the price of the portable GC-FID instruments SRI builds is inflated because the instruments require separate – and pricey – hydrogen generators. That’s a big problem – hydrogen generators are all pretty much the same, and none of them are cheap. This didn’t faze SRI: they just decided to design their own super small on-board hydrogen generator capable of supplying hydrogen to a simple GC macgyversystem for six hours with just 20mL of distilled water from the grocery store! I’m not kidding – I saw it in action on their new Model 420 GC (more on that in some future pieces). Was the final product pretty? Not in the least. Did it work? Absolutely. This kind of MacGyver-esque problem solving can only be done successfully with a deep understanding of the core principles behind the problem. What’s more, in order to engineer instruments like these, SRI has to have mastery over the core principles of not only chromatographic separation, but also of software development, electrical engineering, and mechanical engineering – just to name a few. These quirky, unassuming guys are smart. SRI is a company that’s been unapologetically true to themselves for decades; they’ll never be a contender for beauty queen, but they get the job done.

On the surface, PerkinElmer (PE) contrasts with SRI in almost every way possible. With revenue measured in billions of dollars and employees numbering in the thousands, PE is a behemoth that plays not only in the analytical chemistry industry but also in clinical diagnostics and other large industries. Where SRI instruments have a characteristic look of familiar homeliness, PE instruments are sleek and sexy. However, PerkinElmer and SRI are more alike than it would seem; just like the no-frills SRI, the hyper-technical PE instruments are engineered for a purpose by teams of very smart, passionate people.

DoogieWith its modest price tag and manual sample introduction, the SRI Model 420 is engineered for lower throughput users to be a fast, simple, and inexpensive approach to semi-quantitative process control. The purpose of the instruments manufactured by PE is to produce the highest-quality quantitative results as quickly as possible for high-throughput labs. PE instruments are built using the best technology available in order to eke out every last ounce of quantitative accuracy and throughput possible. Fancy technology is rarely inexpensive, and neither is rigorous product development that can last years in some cases. In a way, PE is Doogie Howser to SRI’s MacGyver. Like MacGyver, Doogie is super smart, and his setting is a sterile hospital rather than a warzone.

I had a wonderful conversation with Tim Ruppel, PE’s headspace-GC specialist, on the sample introduction technology incorporated into the TurboMatrix Headspace Sampler, where I also learned that the basic technology for all PerkinElmer headspace-GC instruments was designed by the men who wrote The Book on headspace gas chromatography: Bruno Kolb and Leslie Ettre**. Later, I was able to get a much-needed lesson on FT-IR and the Spectrum Two IR Spectrometer from Brian Smith, PE’s spectroscopy expert, who actually wrote the book on quantitative spectroscopy***. Tim and Brian’s excitement over their technology mirrored that of Hugh and Greg. It turns out that SRI and PerkinElmer are more alike than I thought.

These two instrument manufacturers have addressed the fast/inexpensive conundrum of cannabis potency testing in two different ways: SRI’s instrument is extremely inexpensive, easy to operate, and will provide semi-quantitative values for THC, CBD, and CBN in just a few minutes; PE’s instrument is more expensive up front, but provides quantitative (though not directly quantitative) values for all of the major cannabinoids almost instantly, and requires almost no maintenance or consumables. These two instruments were designed for specific uses: one for inexpensive, easy use, and the other for more comprehensive results with a higher initial investment. The question consumers have to ask themselves is “Who do I need to solve my problem?” For some, the answer will be MacGyver, and for others, Doogie Howser will provide the solution – after all, both are heroes.


** B. Kolb, L. Ettre, Static Headspace-Gas Chromatography: Theory and Practice, John Wiley & Sons, Hoboken, NJ, 2006.

*** Brian C. Smith, Quantitative Spectroscopy: Theory and Practice, Elsevier, Boston, MA, 2002.

About The Author

amandarigdon

Comments

  1. Rowshan Reordan

    I own Green Leaf Lab. We are a cannabis analytical laboratory that was recently accredited and licensed to test cannabis in Oregon. We used SRI instrumentation when the lab first opened in 2011. Was unimpressed with SRI’s quality of instrumentation and services. Would not recommend them to anyone that is committed to providing quality results or wants to ensure they can get their money out of the instrumentation. I hope that regulators and scientists look towards Oregon for their cannabis regulations. Oregon is leading cannabis science by requiring testing from laboratories that have proper quality systems and technical capabilities for quantitative testing. To legitimize the cannabis industry quantitative testing needs to be a minimum requirement.

  2. Amanda Rigdon

    Hi Rowshan,

    Thanks for your comment. I agree with you one hundred percent that validated, quantitative testing by reputable independent labs should be an absolute requirement for reporting purposes. However, the reason why I introduced both of these approaches is that their purpose is specifically NOT to produce ultra-quantitative results for reporting purposes in a regulatory environment. Instead, these instruments make an excellent tools for producers, growers, and dispensaries – who don’t have the expertise or budget for a quantitative instrument – to easily generate semi-quantitative results in order to control their processes (e.g. “is the extraction finished?”) or to perform confirmatory tests on their products (e.g. “does this bud really have a 2:1 CBD:THC ratio like what’s reported for the whole batch?”). The purpose of this article was definitely not to propose either instrument as a replacement for quantitative chromatographic testing using direct comparison to certified reference materials.

    Now that I’ve re-read the beginning of the article where I talk about HPLC-UV, I can see how the message of this article could be misconstrued. So let me say this definitively: I do not believe that either of the approaches discussed in the article should replace validated HPLC-UV (or MS or MS/MS) methods performed by accredited independent laboratories for reporting purposes.

    I hope this clarifies my point, and I apologize for the confusion and thanks for opening the discussion!

  3. Rowshan Reordan

    Having growers and processors understand their products is important. It is also important for instrument manufacturers to be clear on the fact that the product they are offering is not quantitative and cannot be fully relied upon. Numbers are really important in this industry. If someone gets a result on their flower that is 21% THC from an instrument that they believe to be accurate, and 19% THC from a credentialed laboratory, the customer will be upset. This may create problems for laboratories that are providing quantitative testing and have undergone extensive proofs of their quality systems and technical capabilities.

    1. Hugh Goldsmith

      19 vs 21% implies a variation of about 5% from the average result of 20%. This document from the Colorado Dept of Agriculture http://www.srigc.com/cn/downloads/238/CDA%20Hemp%20Testing%20THC%20Analysis%20Methods.pdf
      indicates that with the very best equipment and experienced operators ( see page 6 ) that they can not achieve that level of accuracy when measuring THC in hemp. They claim around 15%-25% which seems to be about normal. Even the calibration standards from Restek, Cerilliant and Lipomed show more variation than 5% between them. See this doc http://www.srigc.com/cn/downloads/236/CannabinoidStandardsComparisonNov2016.pdf

  4. Matthew Silva

    Matt Silva, chemical engineer at ProVerde Laboratories. I think a place where this article lacks is defining to what “semi-quantitative” is relative. I understand this is supposed to be a less technical article but it should be noted somewhere the main drawback and inaccuracy of GC in general for cannabinoids. GC temperatures will decarboxylate the acid forms of cannabinoids (CBDA, THCA, CBGA, CBCA, etc.) and purport higher THC, CBD, CBG, or CBC values, etc. I think this article could go further in explaining to the reader that the total cannabinoid measurements with GC may actually be rather accurate, but the ratio of acids to neutrals will almost certainly be off every time.

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